Instrumentation
Acknowledgement
Please acknowledge the Proteomics and Mass Spectrometry Core Facilityin publications and talks. It is a requirement of funding agencies and CRUCIAL FOR FUTURE FUNDING.
An RRID is a unique reference tag associated with a specific research resource. Robust metrics tracked by an RRID in publications enable the metrics needed for requesting new instrument funding and promotes meticulous rigor and reproducibility of research results.
The Central Analytical Laboratory facility, located in room C1B90 in the JSC Biotechnology Building, currently houses several Waters UPLCs and five mass spectrometers to provide structural information on various samples (from small organic molecules to large proteins). The facility is available to analyze samples from the both University of Colorado and non-Â鶹Ãâ·Ñ°æÏÂÔØsources.
The following instruments are housed in the facility:
| Manufacturer | Model | Mass Analyzer | Ionization Methods |
|---|---|---|---|
| Shimadzu | MALDI-7090-TOF/TOF | MALDI-TOF/TOF | MALDI |
| Thermo | Orbitrap Q-Exactive HF-X | Quadrupole-Orbitrap | ESI, nanospray |
| Thermo | Orbitrap Exploris 480 | Quadrupole-Orbitrap | ESI, nanospray |
| Thermo | ISQ GCMS | Single Quadrupole | EI |
| Waters | Cyclic IMS | Quadrupole/ToF | ESI |
Our instruments are used to confirm the identity of various organic and inorganic compounds including liquid crystals, carboranes, proteins, peptides, oligonucleotides using both positive and negative ionization modes.
Contact Chris Ebmeier for further details.
Make A Reservation
For any users whoÌýhave been trainedÌýon sample preparation and instrument usage, reservations can be made to allow necessary instrument time.Ìý Currently, we are accepting online reservations for the following instruments only:
- Thermo ISQ GCMS
- Shimadzu MALDI-TOF/TOF
- Waters Cyclic IMS
- Waters HDX nanoUPLC
To access the reservation website using your Identikey + Â鶹Ãâ·Ñ°æÏÂÔØpassword.
Instruments
Waters ACQUITY M-CLASS UPLC PDA
Description:
Waters ACQUITY M-CLASS UPLC with a photodiode array(PDA) detector provides rapid liquid chromatographic separation with UV/visible absorbance detection. It is suitable for qualitative and quantitative analysis of compounds containing UV- or visible-absorbing chromophores. The detector supports single- and dual-wavelength monitoring and uses a low-volume light-guiding flow cell to minimize chromatographic band broadening.
Specifications:
- Wavelength Range: 190–500 nm
- Wavelength Accuracy: ±1.0 nm
- Wavelength Repeatability: ±0.1 nm
- Spectral Bandwidth: <5 nm
- Single-Wavelength Noise: <6 µAU at 230 nm
- Data Acquisition Rate: Up to 80 Hz in single-wavelength mode
- Standard Flow Cell: 100 nL volume, 10 mm path length
- Flow Cell Pressure Limit: 69 bar (1,000 psi)
Waters SELECT SERIES Cyclic IMS QToF ESI
Description:
Waters SELECT SERIES Cyclic IMS is a high-resolution hybrid quadrupole time-of-flight mass spectrometer combining electrospray ionization with cyclic ion-mobility separation. It separates ions according to mass-to-charge ratio and collision cross-section, enabling detailed analysis of isomers, conformers and complex biological mixtures. Multiple passes through the cyclic mobility device provide progressively higher ion-mobility resolution.
Specifications:
- Ionization Source: Electrospray ionization (ESI)
- Mass Analyzer: Hybrid quadrupole/time-of-flight
- Mass Resolution: >100,000 FWHM demonstrated for the 7+ charge state of monomeric streptavidin
- Mass Accuracy: Low-ppm exact-mass measurement
- Single-Pass Ion-Mobility Resolution: Approximately 65 FWHM
- Typical ESI Acquisition Range: m/z 50–2,000, method-dependent
- Ion-Mobility Modes: Single-pass IMS, multipass IMS, IMS/IMS and IMSâ¿
- Mobility Separation: Resolution increases with the number of passes; separations using 100 passes have been demonstrated
- Acquisition Modes: TOF-MS, MS/MS and mobility-enabled HDMSá´±
- Detection System: Dual-gain detection for increased dynamic range
Thermo Scientific Orbitrap Exploris 480 Orbitrap
Description:
Thermo Scientific Orbitrap Exploris 480 is a high-resolution, accurate-mass quadrupole-Orbitrap mass spectrometer. It supports electrospray and nanoelectrospray ionization for qualitative and quantitative analysis of small molecules, metabolites, lipids, peptides and proteins. The system provides precursor selection using a quadrupole, HCD fragmentation and high-resolution Orbitrap detection.
Specifications:
- Ionization Sources: Electrospray ionization (ESI) and nanoelectrospray ionization
- Mass Analyzer: Hybrid quadrupole–ultra-high-field Orbitrap
- Mass Range: m/z 40–6,000; up to m/z 8,000 with the BioPharma option
- Mass Resolution: Up to 480,000 FWHM at m/z 200
- Scan Speed: Up to 40 scans/second at a resolution setting of 7,500
- Mass Accuracy: <1 ppm RMS with internal calibration; <3 ppm RMS with external calibration
- Sensitivity, MS/MS Mode: 50 fg reserpine on-column, 100:1 S/N
- Sensitivity, SIM Mode: 50 fg reserpine on-column, 150:1 S/N
- Nanoelectrospray Flow Range: 50–2,000 nL/min
- Dynamic Range: >5,000 within a single Orbitrap mass spectrum
- Fragmentation: Higher-energy collisional dissociation (HCD)
Thermo Scientific Q Exactive HF-X Orbitrap
Description:
Thermo Scientific Q Exactive HF-X is a high-resolution, accurate-mass hybrid quadrupole-Orbitrap mass spectrometer. It combines quadrupole precursor-ion selection, accelerated higher-energy collisional dissociation and ultra-high-field Orbitrap detection. The system is suitable for proteomics, biopharmaceutical analysis and small-molecule identification and quantification. It supports electrospray ionization and optional nanospray sources. ()
Specifications:
- Ionization Sources: H-ESI II electrospray; optional EASY-Spray and Nanospray Flex
- Mass Analyzer: Hybrid quadrupole–ultra-high-field Orbitrap
- Mass Range: m/z 50–6,000
- Mass Resolution: Up to 240,000 FWHM at m/z 200
- Scan Speed: Up to 40 scans/second at a resolution setting of 7,500
- Mass Accuracy: <1 ppm RMS with internal calibration; <3 ppm RMS with external calibration
- Sensitivity, MS/MS Mode: 100 fg reserpine on-column, 150:1 S/N
- Sensitivity, SIM Mode: 50 fg reserpine on-column, 150:1 S/N
- Dynamic Range: >5,000:1 within a single spectrum
- Precursor Isolation Width: 0.4 Da to the full mass range
- Fragmentation: Accelerated higher-energy collisional dissociation
- Acquisition Modes: Full scan, SIM, PRM, DIA, AIF and data-dependent acquisition
- Polarity Switching: One positive-and-negative scan cycle in under 1 second at 60,000 resolution ()