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Waters ACQUITY M-CLASS UPLC PDA

Description:

Waters ACQUITY M-CLASS UPLC with a photodiode array(PDA) detector provides rapid liquid chromatographic separation with UV/visible absorbance detection. It is suitable for qualitative and quantitative analysis of compounds containing UV- or visible-absorbing chromophores. The detector supports single- and dual-wavelength monitoring and uses a low-volume light-guiding flow cell to minimize chromatographic band broadening.

Specifications:

• Wavelength Range: 190–500 nm
• Wavelength Accuracy: ±1.0 nm
• Wavelength Repeatability: ±0.1 nm
• Spectral Bandwidth: <5 nm
• Single-Wavelength Noise: <6 µAU at 230 nm
• Data Acquisition Rate: Up to 80 Hz in single-wavelength mode
• Standard Flow Cell: 100 nL volume, 10 mm path length
• Flow Cell Pressure Limit: 69 bar (1,000 psi)

Waters SELECT SERIES Cyclic IMS QToF ESI

Description:

Waters SELECT SERIES Cyclic IMS is a high-resolution hybrid quadrupole time-of-flight mass spectrometer combining electrospray ionization with cyclic ion-mobility separation. It separates ions according to mass-to-charge ratio and collision cross-section, enabling detailed analysis of isomers, conformers and complex biological mixtures. Multiple passes through the cyclic mobility device provide progressively higher ion-mobility resolution.

Specifications:

• Ionization Source: Electrospray ionization (ESI)
• Mass Analyzer: Hybrid quadrupole/time-of-flight
• Mass Resolution: >100,000 FWHM demonstrated for the 7+ charge state of monomeric streptavidin
• Mass Accuracy: Low-ppm exact-mass measurement
• Single-Pass Ion-Mobility Resolution: Approximately 65 FWHM
• Typical ESI Acquisition Range: m/z 50–2,000, method-dependent
• Ion-Mobility Modes: Single-pass IMS, multipass IMS, IMS/IMS and IMSⁿ
• Mobility Separation: Resolution increases with the number of passes; separations using 100 passes have been demonstrated
• Acquisition Modes: TOF-MS, MS/MS and mobility-enabled HDMSᴱ
• Detection System: Dual-gain detection for increased dynamic range

Thermo Scientific Orbitrap Exploris 480 Orbitrap

Description:

Thermo Scientific Orbitrap Exploris 480 is a high-resolution, accurate-mass quadrupole-Orbitrap mass spectrometer. It supports electrospray and nanoelectrospray ionization for qualitative and quantitative analysis of small molecules, metabolites, lipids, peptides and proteins. The system provides precursor selection using a quadrupole, HCD fragmentation and high-resolution Orbitrap detection.

Specifications:

• Ionization Sources: Electrospray ionization (ESI) and nanoelectrospray ionization
• Mass Analyzer: Hybrid quadrupole–ultra-high-field Orbitrap
• Mass Range: m/z 40–6,000; up to m/z 8,000 with the BioPharma option
• Mass Resolution: Up to 480,000 FWHM at m/z 200
• Scan Speed: Up to 40 scans/second at a resolution setting of 7,500
• Mass Accuracy: <1 ppm RMS with internal calibration; <3 ppm RMS with external calibration
• Sensitivity, MS/MS Mode: 50 fg reserpine on-column, 100:1 S/N
• Sensitivity, SIM Mode: 50 fg reserpine on-column, 150:1 S/N
• Nanoelectrospray Flow Range: 50–2,000 nL/min
• Dynamic Range: >5,000 within a single Orbitrap mass spectrum
• Fragmentation: Higher-energy collisional dissociation (HCD)

Thermo Scientific Q Exactive HF-X Orbitrap

Description:

Thermo Scientific Q Exactive HF-X is a high-resolution, accurate-mass hybrid quadrupole-Orbitrap mass spectrometer. It combines quadrupole precursor-ion selection, accelerated higher-energy collisional dissociation and ultra-high-field Orbitrap detection. The system is suitable for proteomics, biopharmaceutical analysis and small-molecule identification and quantification. It supports electrospray ionization and optional nanospray sources. ()

Specifications:

• Ionization Sources: H-ESI II electrospray; optional EASY-Spray and Nanospray Flex
• Mass Analyzer: Hybrid quadrupole–ultra-high-field Orbitrap
• Mass Range: m/z 50–6,000
• Mass Resolution: Up to 240,000 FWHM at m/z 200
• Scan Speed: Up to 40 scans/second at a resolution setting of 7,500
• Mass Accuracy: <1 ppm RMS with internal calibration; <3 ppm RMS with external calibration
• Sensitivity, MS/MS Mode: 100 fg reserpine on-column, 150:1 S/N
• Sensitivity, SIM Mode: 50 fg reserpine on-column, 150:1 S/N
• Dynamic Range: >5,000:1 within a single spectrum
• Precursor Isolation Width: 0.4 Da to the full mass range
• Fragmentation: Accelerated higher-energy collisional dissociation
• Acquisition Modes: Full scan, SIM, PRM, DIA, AIF and data-dependent acquisition
• Polarity Switching: One positive-and-negative scan cycle in under 1 second at 60,000 resolution ()